Bromocresol purple or 5′,5″-dibromo-o-cresolsulfophthalein is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
In microbiology Bromocresol Purple is used as a component in Bromocresol Purple Lactose Agar. It is a differential, non-selective medium for use in enumerating bacteria in urine. It supports the growth of urinary pathogens and contaminants but prevents undue swarming of Proteus species due to its lack of electrolytes. The medium allows
the differentiation of the bacteria recovered into lactose fermenters and non-fermenters.
Dyes, Stains & Indicators
|Dye content min 87%
|Grey to light pink to purple powder
|MSDS Data Sheet
|Click to download
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